Coding
YFP:tetR

Part:BBa_K606025:Design

Designed by: K�vin Yauy   Group: iGEM11_Paris_Bettencourt   (2011-07-27)

YFP:tetR recombinant fusion protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 631
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

1 Quick Change Mutagenesis and 1 mutation on the primers has been done

Source

Andrew Wright, Dave Lane, François-Xavier Barre, pFX234 plasmid.

References

Chromosome and Low Copy Plasmid Segregation in E. coli: Visual Evidence for Distinct Mechanisms, G. Scott Gordon et al, Cell, 1997
Kinetics of plasmid segregation in Escherichia coli, G. Scott Gordon et al, Molecular Microbiology, 2004